Thaumatin (Amanful et al., 2024)

Technical Data

Microorganism name	Komagatella phaffi (Pichia pastoris)
Target proteins	Thaumatin (Amanful et al., 2024)
Wild-type or GMO	GMO (Amanful et al., 2024)
Production mode (intracellular/extracellular)	Extracellular (Amanful et al., 2024)
Protein yield (g/L or g/g?)	0.03 g/L (Amanful et al., 2024)
Temperature used in study	28°C (Amanful et al., 2024)
pH used in study	NA
C & N source	Molasses, BMG medium (Amanful et al., 2024)
Regulatory status in Europe	Not allowed
Regulatory status in other parts of the world	No FDA GRAS approval in US, not allowed in Canada
Companies	NA
Publications/references	Amanful, B., Dogbe, E. S., Bosman, C. E., & Görgens, J. F. (2024). Stochastic techno-economic analysis for the co-production of alternative sweeteners in sugarcane biorefineries. Food and Bioproducts Processing, 143, 9–20. https://doi.org/10.1016/j.fbp.2023.10.006 Spohner, S. C., Müller, H., Quitmann, H., & Czermak, P. (2015). Expression of enzymes for the usage in food and feed industry with Pichia pastoris. Journal of Biotechnology, 202, 118–134. https://doi.org/10.1016/j.jbiotec.2015.01.027 Lv, W., & Cai, M. (2025). Advancing Recombinant Protein Expression in Komagataella phaffii: Opportunities and Challenges. FEMS Yeast Research. https://doi.org/10.1093/femsyr/foaf010 Pan, Y., Yang, J., Wu, J., Yang, L., & Fang, H. (2022). Current advances of Pichia pastoris as cell factories for production of recombinant proteins. Frontiers in Microbiology, 13. https://doi.org/10.3389/fmicb.2022.1059777 Barone, G. D., Emmerstorfer-Augustin, A., Biundo, A., Pisano, I., Coccetti, P., Mapelli, V., & Camattari, A. (2023). Industrial Production of Proteins with Pichia pastoris—Komagataella phaffii. Biomolecules, 13(3), 441. https://doi.org/10.3390/biom13030441 Ergün, B. G., Laçın, K., Çaloğlu, B., & Binay, B. (2022). Second generation Pichia pastoris strain and bioprocess designs. Biotechnology for Biofuels and Bioproducts, 15(1). https://doi.org/10.1186/s13068-022-02234-7